ONE GEL PAGE PLUS KIT ์ถœ์‹œ๐Ÿ˜

์•ˆ๋…•ํ•˜์„ธ์š” ์„ ์ƒ๋‹˜ ISS์ž…๋‹ˆ๋‹ค. ์˜ค๋Š˜์€ sds pagegel ์œ ์ €๋ถ„๋“ค์—๊ฒŒ ์ข‹์€ ์ •๋ณด๋ฅผ ์•Œ๋ ค๋“œ๋ฆด๋ ค๊ณ  ํ•ฉ๋‹ˆ๋‹ค. ํ˜„์žฌ ํŒ๋งค ์ค‘์ธ One Gel PAGE Kit ๋Š” ๋ณธ ์‚ฌ์˜ ๋…์ž์ ์ธ ๊ธฐ์ˆ ๋กœ ๊ฐœ๋ฐœ๋œ ์ œํ’ˆ์ž…๋‹ˆ๋‹ค. ๊ตญ๋‚ด์—์„œ ํŒ๋งค๋˜๊ณ  ์žˆ๋Š” ์ œํ’ˆ๋“ค ์ค‘์—๋Š” ๋ณธ ์ œํ’ˆ๊ณผ ์œ ์‚ฌํ•œ ์ œํ’ˆ์€ ์—†์Šต๋‹ˆ๋‹ค. ๊ฐ€์žฅ ๋น„์Šทํ•œ ์ œํ’ˆ์€ Fisher scientific ์ œํ’ˆ ์ค‘ EZ-Run Protein Gel Solution์ด ์žˆ์ง€๋งŒ ๊ตญ๋‚ด์—์„œ ๋งŽ์ด ์‚ฌ์šฉ๋˜๊ณ  ์žˆ๋Š” ๊ฒƒ ๊ฐ™์ง€๋Š” ์•Š์Šต๋‹ˆ๋‹ค.

๋ณธ ์ œํ’ˆ์€ SDS-PAGE ์— ์‚ฌ์šฉํ•˜๋Š” Gel์„ ๋งŒ๋“œ๋Š” solution์ž…๋‹ˆ๋‹ค. ์ง€๊ธˆ๊นŒ์ง€๋Š” ๋‹จ๋ฐฑ์งˆ์„ ์ „๊ธฐ์˜๋™ํ•˜๊ธฐ ์œ„ํ•ด์„œ Gel์„ ๋งŒ๋“ค ๋•Œ๋Š” Resolving gel์„ ์œ„ํ•ด์„œ Acrylamide-Bis solution, 1.5M Tris-HCl pH 8.8, SDS solution, APS, TEMED Stacking Gel์„ ์œ„ํ•ด์„œ Acrylamide-Bis solution, 0.5M Tris-HCl pH 8.8, SDS solution, APS, TEMED ๊ฐ€ ํ•„์š”ํ–ˆ์Šต๋‹ˆ๋‹ค. ๊ทธ๋Ÿฐ๋ฐ ๋ณธ ์ œํ’ˆ์€ Solution A์™€ B, APS,TEMED๋กœ ๊ตฌ์„ฑ๋˜์–ด ์žˆ์–ด SDS-PAGE๋ฅผ ์œ„ํ•ด์„œ resolving gel๊ณผ stacking gel์„ ๋”ฐ๋กœ ๋งŒ๋“ค ํ•„์š”์—†์ด Solution A์™€ B๋ฅผ ๋™๋Ÿ‰์œผ๋กœ ์„ž์€ ํ›„ APS์™€ TEMED๋ฅผ ๋„ฃ์–ด ์ „๊ธฐ์˜๋™ํ•˜๋ฉด ๋ฉ๋‹ˆ๋‹ค. ์ „๊ธฐ์˜๋™ ํ›„์—๋Š” ์—ผ์ƒ‰์„ ํ•˜๊ฑฐ๋‚˜ western blot์— ํ™œ์šฉํ•˜์‹ค ์ˆ˜ ์žˆ์Šต๋‹ˆ๋‹ค. ์—ฌ๋Ÿฌ ์‹œ์•ฝ์„ ์ค€๋น„ํ•ด์„œ ์‹คํ—˜ํ•˜๋Š” ๋ถˆํŽธํ•จ์„ ํ•ด์†Œํ•˜๊ณ  ๊ฐ„๋‹จํ•˜๊ณ  ์‰ฝ๊ฒŒ ์‹คํ—˜ํ•  ์ˆ˜ ์žˆ๋„๋ก ํ•˜๋Š” ์ œํ’ˆ์ž…๋‹ˆ๋‹ค. ํ˜„์žฌ 7.5%, 10%, 12%, 15%, Master ver.์ด ์ถœ์‹œ๋˜์—ˆ์Šต๋‹ˆ๋‹ค. ์ƒ˜ํ”Œ์„ ์š”์ฒญํ•˜์‹œ๋ฉด ์ œ๊ณตํ•ด๋“œ๋ฆฝ๋‹ˆ๋‹ค. ๊ทธ ๋ฐ–์— ๋ฌธ์˜์‚ฌํ•ญ ์žˆ์œผ์‹œ๋ฉด ๋‹ค์‹œ ์—ฐ๋ฝ์ฃผ์‹ญ์‹œ์š”

๊ฐ„๋‹จํ•œ ์‚ฌ์šฉ๋ฒ•์ž…๋‹ˆ๋‹ค~

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